Abstract

AbstractUnder laboratory conditions (L16:D8, 24°C, r.h. 80%), applying of an ecdysone agonist, RH 5849, (1,2‐dibenzoyl‐1‐tert‐butylhydrazine) onto final instar larvae of Spodoptera littoralis (Boisd.), Noctuidae, Lepidoptera, delayed or advanced the time of pupation depending on the time of treatment. This response was dose‐dependent. In addition, non‐lethal doses of RH 5849, ranging from 0.05 to 10 μg/larva were used. Following treatment of S. littoralis larvae with 5 μg RH 5849 the endogenous ecdysteroid level was increased or decreased depending on the time of application. Based on the results presented, we suggest that potential users of non‐steroidal ecdysone agonists should consider the proper circadian timing of application in the field.

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