Abstract
Within the globus pallidus, CB1 cannabinoid receptors are localized presynaptically on GABAergic neurons. We focused on effect of endocannabinoids on GABA uptake in rat globus pallidus slices. Following incubation with 2‐arachidonyl glycerol (2‐AG) (1 and 3 μm), there was a 40%, and 30% increase, respectively, in [3H]‐GABA uptake (P < 0.05). When incubated with noladin ether (1 μm), an increase of 57% was observed (P < 0001). The effects of 2‐AG were reversed by the CB1 receptor antagonist AM 251 (1 μm), showing this mechanism is likely to be CB1 receptor‐mediated (P < 0.05). Neither anandamide (0.1–10 μm) nor synthetic cannabinoid WIN 55,212–2 (1–100 μm) had any effect. We suggest increased level of endocannabinoids, found in a Parkinson's disease model, might thus represent a compensatory mechanism.Acknowledgement: Supported by CEZ:J13/98:11600004.
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