End-of-day Far-red Lighting with a Low Daily Light Integral Increases Stem Length But Does Not Promote Early Leaf Expansion for Petunia ×hybrida Seedlings

Abstract

Greenhouse production of high-quality young annual bedding plants (plugs) at northern latitudes often requires supplemental lighting to compensate for a low natural daily light integral (DLI), but radiation interception by plugs is limited by a low leaf area index. Some species show an increase in leaf area in response to growth under a low ratio of red to far-red radiation (R:FR), and an early increase in leaf area may allow for more effective radiation capture by seedlings and a reduction in wasted radiation. Thus, the objective of this study was to examine the effects of end-of-day far-red (EOD-FR) radiation treatments varying in intensity, R:FR (600–700 nm/700–780 nm), and duration on early leaf expansion and plug quality for petunia (Petunia ×hybrida) ‘Wave Purple’ and ‘Dreams Midnight’. Seedlings were grown in 128-cell trays in a common greenhouse environment under a simulated winter DLI (∼5.3 mol·m−2·s−1) and received one of four EOD-FR treatments, control conditions (no EOD-FR or supplemental lighting), or supplemental lighting (target photosynthetic photon flux density of 70 μmol·m−2·s−1). The EOD-FR treatments were provided for 3 weeks on cotyledon emergence and included the following: 10 μmol·m−2·s−1 of far-red radiation for 30 minutes with a R:FR of ∼0.8 (EODFL), 10 or 20 μmol·m−2·s−1 of far-red radiation for 30 minutes with a R:FR of ∼0.15 (EOD10:30 and EOD20:30, respectively), or 20 μmol·m−2·s−1 of far-red radiation for 240 minutes with a R:FR of ∼0.15 (EOD20:240). Destructive data were collected 14 and 21 days after cotyledon emergence. Seedlings that received EOD-FR treatments did not show any increase in leaf area compared with control or supplemental lighting treatments. Stem length generally increased under EOD-FR treatments compared with supplemental lighting and control treatments; greater elongation was observed when the R:FR decreased from 0.8 to 0.15, and when treatment duration increased from 30 minutes to 240 minutes. However, at a R:FR of 0.15 and a treatment duration of 30 minutes, an increase in far-red radiation intensity from 10 to 20 μmol·m−2·s−1 did not promote further stem elongation resulting in similar stem lengths for both cultivars under EOD10:30 and EOD20:30. Results of this study indicate that under low DLIs, EOD-FR radiation applied in the first 3 weeks of seedling production does not promote early leaf area expansion, and generally decreases seedling quality for petunia. As responses to far-red radiation may vary based on study taxa, incident radiation, and DLI, future research examining EOD-FR–induced morphological changes is warranted.