Encoding function into polypeptide-oligonucleotide precision biopolymers.

Weina Liu,Yuzhou Wu,Bikram Keshari Agrawalla,Tao Wang,Yu Tokura,Felix Boldt,Tanja Weil

doi:10.1039/c8cc04725a

Abstract

We report a novel synthesis strategy to prepare precision polymers providing exact chain lengths, molecular weights and monomer sequences that allow post modifications by convenient DNA hybridization. Two grafted single strand DNA (ssDNA) side chains serve as a versatile platform for sequence-specific attachment of chromophores, proteins, cell-targeting peptide, and a Y-shape DNA linker. This approach resembles a LEGO®-type incorporation of functionalities to create functional biopolymers of high structure definition under mild conditions.

Highlights

Weina Liu,‡ab Felix Boldt,‡ab Yu Tokura,ab Tao Wang,bc Bikram Keshari Agrawalla,ab Yuzhou Wu*ad and Tanja Weil *ab
We report a novel synthesis strategy to prepare precision polymers providing exact chain lengths, molecular weights and monomer sequences that allow post modifications by convenient DNA hybridization
Two grafted single strand DNA side chains serve as a versatile platform for sequence-specific attachment of chromophores, proteins, cell-targeting peptide, and a Y-shape DNA linker

Summary

Introduction

Weina Liu,‡ab Felix Boldt,‡ab Yu Tokura,ab Tao Wang,bc Bikram Keshari Agrawalla,ab Yuzhou Wu*ad and Tanja Weil *ab. It serves as a defined platform with high molecular weight (66.3 kDa) and distinct amino acid sequence that allow various post-modifications.[9] The globular structure of HSA can be unfolded by protein denaturation followed by grafting polyethylene(oxide) (PEO) side chains that prevent uncontrolled aggregation and precipitation and retain the polypeptide main chain in solution.

Results

Conclusion