Abstract
Steady state absorption and fluorometric techniques have been used to investigate the photophysics of norharmane (NHM), a bioactive fluorophore, in aqueous as well as aqueous cyclodextrin (CD) environments. The absorption and steady state fluorescence spectral studies reveal the formation of two types of inclusion complexes between the fluorophore and β-cyclodextrin (β-CD) depending on the relative population of the two. The stoichiometries and association constants of these complexes have been determined monitoring the fluorescence data. α-and γ-cyclodextrin (α-CD, γ-CD) do not have appreciable effect on the spectral pattern of the fluorophore. The differential fluorimetric behavior of NHM in different CD environments has been rationalized from the variation of the relative dimensions of the probe and the CD cavities.
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Schedule a callMore From: Journal of Photochemistry & Photobiology, B: Biology
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