Encapsulated VEGF121-PLA microparticles promote angiogenesis in human endometrium stromal cells

Sunil Abraham,Geetha Sanjay,Noushin Abdul Majiyd,Amutha Chinnaiah

doi:10.1186/s43141-021-00118-1

Abstract

BackgroundIn this study, Vascular Endothelial Growth Factor 121 expressed abundantly in endometrial stromal cells is encapsulated with poly-l-lactide and characterized the properties for endometrial angiogenesis. We studied the migration, proliferation and the protein levels of human immortalized endometrium stromal cells after treating the cells with recombinant Vascular Endothelial Growth Factor (200 and 500 nanogram), and poly-l-lactide loaded Vascular Endothelial Growth Factor 121 (day 1, 20 and 30). The present study explains endometrium angiogenesis because endometrium plays an important role in pregnancy. ResultsMigration and proliferation studies in endometrium cells proved the efficiency of Vascular Endothelial Growth Factor and poly-l-lactide loaded Vascular Endothelial Growth Factor 121. This proliferated and increased the migration of the cells in vitro and also activated the Protein kinase B, Phosphatidylinositol-4, 5-Bisphosphate 3-Kinase Catalytic Subunit Beta, α-Smooth muscle actin and vascular endothelial growth factor receptor 2 pathways. Western blot analysis showed the increased expression levels of kinases, smooth muscle actin and vascular endothelial growth factor receptor 2 after the treatment with Vascular Endothelial Growth Factor and poly-l-lactide loaded Vascular Endothelial Growth Factor 121 particles in comparison to the control group. The elevated levels of α-Smooth muscle actin in endometrium cells with Vascular Endothelial Growth Factor prove the regulation of angiogenesis in vitro. ConclusionEndometrium thickness is one of the important factors during implantation of embryo and pregnancy. Slow release of VEGF from PLA encapsulated microparticle further controls the endothelial cell proliferation and migration and helps in the promotion of angiogenesis. The combined effect studied in vitro could be used as a pro-angiogenic drug on further in vivo confirmation.

Highlights

In this study, Vascular Endothelial Growth Factor 121 expressed abundantly in endometrial stromal cells is encapsulated with poly-l-lactide and characterized the properties for endometrial angiogenesis
Proliferation % of human endometrium stromal cell lines (HESC) cells was 38.22% in 200 ng of rVEGF121 and 46.12% in the cells induced with 500 ng of r VEGF121, while 57.26% increase was observed in the positive control
Cells treated with Vascular endothelial growth factor (VEGF)-PLA microparticles at different time points showed significant (p < 0.0001) proliferative effects in HESC (Fig. 2b) such as 41.12%, 17.86%, and 12.45% respectively when compared with Phosphate Buffered Saline (PBS)-loaded PLA microparticles (3.80%)

Summary

Introduction

Vascular Endothelial Growth Factor 121 expressed abundantly in endometrial stromal cells is encapsulated with poly-l-lactide and characterized the properties for endometrial angiogenesis. Proliferation and the protein levels of human immortalized endometrium stromal cells after treating the cells with recombinant Vascular Endothelial Growth Factor (200 and 500 nanogram), and poly-l-lactide loaded Vascular Endothelial Growth Factor 121 (day 1, 20 and 30). The inner lining of the uterus is called endometrium, and it supports and nourishes the developing baby. A new vascular system develops in the endometrium through the process called angiogenesis which supports cell growth and differentiation [1]. The functional layer of the endometrium is differentiated into the glandular epithelium, luminal epithelium, stroma, and vascular compartments [2]. Stromal cells undergo decidualization in the presence of estrogen and progesterone [2]

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Conclusion