Abstract
Simultaneous enantioseparation of three chiral triazole fungicides i.e. penconazole, myclobutanil and triadimefon bycyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) under acidic condition was investigated withdifferent HP--CD concentrations as chiral selector. The three triazole fungicides have an asymmetrically substitutedcarbon atom respectively, and they all contain two enantiomers. In order to achieve the best enantioresolution of thesetriazole fungicides, the HP--CD concentration range was optimized from 0 to 40 mM in 10 mM increment. The resultsindicate that separation of enantiomers was not achieved with concentration range of HP--CD from 0 to 20 mM.Simultaneous enantioseparation was successfully achived at 30 mM and 40 mM HP--CD concentration in 25 mMphosphate buffer (pH 3.0) solution that containing 50 mM SDS, 10% methanol and 5% acetonitrile. However,enantioresolution decreased for myclobutanil and triadimefon enantiomers with HP--CD concentration greater than 30mM. In addition, an increase in the concentration of HP--CD caused a significant increase in the analysis time. In light ofthese aspects, the optimal concentration for HP--CD was decided to be 30 mM for the simultaneous chiral separation ofthe three triazole fungicides with resolutions (Rs) between enantiomers for penconazole, Rs = 0.81; myclobutanil, Rs = 1.14;triadimefon, Rs = 2.09; peak efficiencies (N) greater than 108 000 for all stereoisomers and analysis time of less than 15min.
Highlights
The present paper investigates influence of hydroxypropyl-γ-cyclodextrin (HP-γ-CD) concentration on chiral separation of three chiral triazole fungicides i.e. penconazole, myclobutanyl and triadimefon by cyclodextrinmodified micellar electrokinetic chromatography (CD-MEKC)
Simultaneous enantioseparation of three chiral triazole fungicides i.e. penconazole, myclobutanil and triadimefon by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) under acidic condition was investigated with different HP-γ-CD concentrations as chiral selector
The results indicate that separation of enantiomers was not achieved with concentration range of HP-γ-CD from 0 to 20 mM
Summary
The present paper investigates influence of hydroxypropyl-γ-cyclodextrin (HP-γ-CD) concentration on chiral separation of three chiral triazole fungicides i.e. penconazole, myclobutanyl and triadimefon by cyclodextrinmodified micellar electrokinetic chromatography (CD-MEKC). The HP-γ-CD is a nonionic cyclic oligosaccharides consisting of eight glucose units and has numerous chiral recognition centers. It is a derivatized neutral cyclodextrin and widely used as chiral selector due to the water solubility and low UV absorbance. It has been used as chiral selector in the CD-MEKC methods [1,2,3,4]. In our previous work [4], the optimized CD-MEKC method for the separation of two enantiomeric pairs of propiconazole was achieved. The use of a mixture of 30 mM HP-γ-CD, 50 mM SDS and methanol-acetonitrile 10%:5% (v/v) in 25 mM phosphate buffer solution was able to separate two enantiomeric pairs of propiconazole with resolution (Rs) greater than 1.50, peak efficiencies (N) greater than 400 000 and analysis time of less than 16 min
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