Enantioselective separation of phenylglycidates by capillary electrophoresis employing sulfated β-cyclodextrin as chiral selector

Abstract

A capillary electrophoresis (CE) method for the enantioseparation of phenylglycidates has been developed. Successful enantioseparation was achieved using sulfated β-cyclodextrin as chiral selector in a phosphate buffer. The effects of varying pH, sulfated β-cyclodextrin concentration and electrophoresis voltage were systematically investigated and the optimized separation conditions were thus obtained. When the migration time was set at the threshold value, it was found that the best enantioseparation was obtained at 10 kV with 3% (w/v) sulfated β-cyclodextrin at pH 6.5. A range of substituted phenylglycidates were successfully separated using the method and the results shown to be superior to those obtained using gas chromatography (GC).