Enantioselective effects of cyflumetofen on microbial community and related nitrogen cycle gene function in acid-soil

Abstract

Cyflumetofen (CYF) is a novel chiral acaricide widely used in commercial crops to control mites. The environmental risks exposed by CYF in the soil, especially at the enantiomer level, remain unclear. We found that the (+)-CYF enantiomer was preferentially degraded in acid-soil, resulting in (−)-CYF enrichment. 16S rRNA and qPCR analysis indicated that decreased bacterial abundance by 12.79–61.80% and 2.52–52.48% in (−)-CYF treatment and (+)-CYF treatment, respectively. Diversity was also decreased with (−)-CYF treatment. Interestingly, several beneficial bacteria, for instance, Alphaproteobacteria (class), Sphingomonadaceae (family), and Arthrobacter (specise) were more enriched following (−)-CYF. The abundance of N2-fixing bacteria showed a sustained reduction with time, and the decrease was 3.24–72.94% with (−)-CYF and 25.37–73.11% with (+)-CYF treatment. Compared with the (+)-CYF treatment could positively promote nitrification, while the treatment (−)-CYF significantly reduced the abundance of amoA gene; namely it significantly negatively affected the nitrification in the nitrogen cycle. Through our further research, we found that Actinobacteria, Alphaproteobacteria, Lysobacter; Sphingomonas, Patescibacteria, Saccharimonadia, and Saccharimonadales showed synergistic effects with the nitrogen cycling-related genes nifH and amoA. These results contribute to a comprehensive environmental risk assessment of CYF in acid-soil at the enantiomer level.