Enantioselective degradation of prothioconazole in soil and the impacts on the enzymes and microbial community

Abstract

In this work, the stereoselective degradation of prothioconazole in five soils was investigated and the metabolite prothioconazole-desthio was determined. The effects of prothioconazole on soil enzymes activities and microbial community were also studied. The dissipation of prothioconazole fitted with a first-order kinetic equation with half-lives ranging from 3.45 to 9.90 days. In addition, R-prothioconazole degraded preferentially than S-prothioconazole in all soils with EF values >0.5. Prothioconazole-desthio formed rapidly with preference in R-enantiomer, and the concentration kept at a considerable level even at the end of the incubation, indicating it was relatively persistent in soil. Prothioconazole and its metabolite inhibited the activity of dehydrogenase, catalase and urease in soils, and could affect the diversity of the soil microbiota as well. Redundancy analysis (RDA) and Spearman analysis showed the abundance of Proteobacteria, Fusobacteria, Firmicutes, Thaumarchaeota, Saccharibacteria, Chloroflexi, Chlorobi, Actinobacteria and Nitrospirae might be related to the enantioselective degradation. The work was helpful for understanding the environmental behavior of the fungicide prothioconazole and its primary metabolite on an enantiomeric level.