Enantioselective bioaccumulation and toxicity of the novel chiral antifungal agrochemical penthiopyrad in zebrafish (Danio rerio)

Abstract

Succinate dehydrogenase inhibitor (SDHI) fungicides has been extensively used in agricultural production, which are not easily degrade in the environment and have various toxic effects on aquatic organisms. However, the toxic effects information to non-target organisms were mostly at the racemate level, which were poorly understood at the enantiomers level. Thus, this study aimed to investigate the enantioselective bioaccumulation behavior and toxic effects of penthiopyrad in zebrafish. Significant enantioselective bioaccumulation was observed when exposed to penthiopyrad at two dose levels: S-(+)-penthiopyrad was preferentially accumulated. Moreover, S-(+)-penthiopyrad caused oxidative stress in zebrafish liver. The results of real-time RT-PCR analyses revealed that exposure to penthiopyrad also enantioselectivity interfered with the expression of mitochondrial respiratory complexes, mtDNA synthesis, lipid metabolism and apoptosis-related genes. S-(+)-penthiopyrad significantly decreased most of the expression of the above gene, which showed higher toxic effects. We inferred that the toxicity mechanism of penthiopyrad was caused by lipid metabolism disorder and mitochondrial dysfunction in zebrafish, and further leads to apoptosis even DNA damage. This study provides more accurate data to investigate the environmental impact of penthiopyrad at the enantiomer level.