Abstract

Enantioselective binding interaction of the pesticides, metolachlor (RAC-metolachlor) and its S-enantiomer (S-metolachlor), with bovine serum albumin (BSA) was investigated by fluorescence and UV–vis absorption spectroscopy. Both RAC- and S-metolachlors quenched the intrinsic fluorescence of BSA via a static mechanism, and various binding parameters indicated that electrostatic forces were involved in the binding of both of these compounds. Site marker competitive experiments demonstrated that S-metolachlor bound to site I of BSA, while R-metolachlor bound to site II, indicating the importance of enantiomeric factors for binding site selection. Further experiments showed that S-metolachlor had a higher binding affinity to BSA than R-metolachlor. The obtained spectral data were resolved with use of the multivariate curve resolution-alternating least squares method (MCR-ALS), and the extracted concentration profiles of the reacting species in the interaction were obtained. These profiles indicated that S-metolachlor was the main active constituent of RAC-metolachlor for binding with BSA, and these findings have significant implications in providing an explanation why S-metolachlor is the preferred herbicide in practice than RAC-metolachlor.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call