Abstract
Two methods for the direct separation of the enantiomers of trimetoquinol hydrochloride, denopamine and timepidium bromide were investigated, namely capillary zone electrophoresis (CZE) using cyclodextrins (CDs) (CD-CZE) and high-performance liquid chromatography (HPLC) using chiral stationary phases (CSPs). By CZE, separation of the enantiomers of trimetoquinol was successfully achieved when using β-cyclodextrin (β-CD), heptakis(2,6-di-0-methyl)-β-cyclodextrin (DM-β-CD) and β-CD polymer, but direct HPLC separation of the enantiomers was not successful. The enantiomers of denopamine were successfully separated by both CZE, employing DM-β-CD under acidic conditions, and HPLC, with cyclodextrin immobilized CSPs (CD-CSPs). Timepidium bromide, which is a racemic drug, was successfully enantioseparated by both CD-CZE and HPLC with CD-CSPs. The CD-CZE mode was selected as the optical purity testing method for trimetoquinol and denopamine because of the superiority of capillary electrophoretic (CE) techniques. Method validation results obtained clearly show that the CE method would be useful as an quality control method for assessing the optical purity of the drugs.
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