Enantiomeric separation of amphetamine and phenylephrine by cyclodextrin-mediated capillary zone electrophoresis

Abstract

The enantiomeric separation of amphetamine and phenylephrine was investigated using cyclodextrin (CD)-mediated capillary zone electrophoresis. The enantiomers of amphetamine can be baseline separated by using 50 m M Tris-H 3PO 4 buffer (pH 2.3) containing 12 m M heptakis(2,6-di-O-methyl)-β-CD and those of phenylephrine by using 50 m M Tris-H 3PO 4-6 M urea-100 m M β-CD (pH 2.3) buffer. CD type and concentration, buffer composition, organic modifier and capillary length all play a role in chiral separation.