Abstract
Capillary electrophoresis has been used for the enantiomeric resolution of several basic compounds of pharmaceutical interest employing a background electrolyte containing pepsin as chiral selector. The separations have been performed in a polyacrylamide-coated capillary to suppress or minimize the electroosmotic flow as well as the adsorption on the wall. The chiral selector filled only part of the capillary, moving in the opposite direction of analytes and keeping the detector cell free of the strong absorbing protein. The effect of pepsin concentration, pH, and concentration of the background electrolyte on resolution of enantiomers was studied. Enantiomeric resolution was achieved for trimipramine, propranolol, promethazine, verapamil, cloperastine, oxprenolol, and pindolol. © 1997 John Wiley & Sons, Inc. J Micro Sep9: 9–14, 1997
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
