Abstract
The direct enantiomeric resolution of five O-aryl O-alkyl N-alkyl-phosphoramidothioates was carried out and their pure enantiomers were prepared on a commercially available Pirkle model chiral column (OA-4700). Absolute configurations of the enantiomers were determined by circular dichroism (CD) spectroscopy. S-enantiomers were the first compounds to elute chromatographically and the chromatographic elution order was in accordance with the results of computer simulating calculation. The biological activity test suggested that all the S-enantiomers were more active than R-enantiomers in killing barnyard grass.
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