Enantiomer Resolution of Nonsteroidal Anti-Inflammatory Drugs on Chiral Stationary Phases Derived from Polysaccharide Derivatives

Abstract

Abstract The liquid chromatographic enantiomerPDF separation of nonsteroidal anti-inflammatory drugs (NSAIDs) was performed on covalently immobilized chiral stationary phases (CSPs) (Chiralpak IA, Chiralpak IB, and Chiralpak IC) and coated-type CSPs (Chiralpak AD and Chiralcel OD) derived from polysaccharide derivatives. The chromatographic conditions were as follows: the flow rate was 1.0 ml min−1, the detection wavelength was 254 nm, and the standard mobile phase was 2-propanol/hexane/trifluoroacetic acid on all CSPs. A mobile phase of 2-propanol/hexane/trifluoroacetic acid = 10:90:0.1 (v/v) and 2:98:0.1 (v/v) were used on Chiralpak AD and Chiralcel OD of coated-type CSPs, respectively. Several solvents (dichloromethane, tetrahydrofuran, and ethyl acetate) in the mobile phase were used on covalently immobilized CSPs (Chiralpak IA, Chiralpak IB, and Chiralpak IC) and, therefore, solvent versatility of the covalently immobilized CSPs for enantiomer separation of NSAIDs was shown. Also, chromatographic comparisons for enantiomer resolution of these analytes were made on amylose tris (3,5-dimethylphenylcarbamate) derived CSPs (Chiralpak IA and Chiralpak AD) and cellulose tris (3,5-dimethylphenylcarbamate) derived CSPs (Chiralpak IB and Chiralcel OD), respectively. The results indicated that Chiralpak IA and Chiralpak IB showed generally lower enantiomer separation than Chiralpak AD and Chiralcel OD, respectively.