Abstract
A continuous turbidimetric method for the discrimination of enantiomers (L- and D-lysine) by the inhibitory action of L-lysine on the crystallization of L-glutamic acid is proposed. A multidetection flow system including an open-closed loop and a single detector permits the determination of kinetic parameters for the crystallization of L-glutamic acid in the presence of 2-propanol. L-Lysine can thus be determined in the presence of a 20 times higher D-lysine concentration. The proposed method was applied to the determination of L-lysine in pharmaceutical preparations with good results.
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