Enamel Distribution in 3D: Is Enamel Thickness More Uneven in the Upper Second Molars of Durophagous Hominoids?

Abstract

Enamel thickness is not uniform across the dental crown of primates. It has been suggested that enamel distribution could be used in taxonomy or for ecological inferences. For instance, the thickness of molar enamel in mammals consuming hard food is expected to be uneven, despite differing reports on extant and extinct apes. Overall estimations of average and relative enamel thickness may mask the details of enamel distribution in complex teeth such as molars. Investigating enamel distribution and its purported relationship with ecology or phylogeny would require more detailed assessments. This paper aims to assess whether apes that consume hard foods on a regular basis, such as Pongo pygmaeus, can be characterized by the evenness or unevenness of enamel thickness. To do so, we combined topographic maps and distribution histograms of enamel thickness with cumulative profiles of its variation, or “pachymetric profiles”. We investigated a sample of 25 unworn hominoid upper second molars scanned by X-ray microtomography, and further compared this to a sample of 32 cercopithecines and colobines. Topographic maps show uniformly thin enamel for Gorilla gorilla and Hylobates sp., unevenly thin enamel for Pan paniscus and Pan troglodytes, and unevenly thick enamel for Pongo pygmaeus. The skewness of enamel distribution does not distinguish between ape species, but does separate apes from OldWorld monkeys. Contrary to previous reports on OldWorld monkeys, the slope of enamel thickness profiles, or pachymetric slope, does not predict the diet of extant apes. However, it does separate the Pan genus, which is characterized by a higher pachymetric slope indicating more uneven enamel distribution compared to other apes. The uneven thickness of enamel distribution observed on topographic maps for P. pygmaeus is not supported by its low pachymetric slope, which instead indicates uniform enamel distribution. This discrepancy in the results obtained for P. pygmaeus can be interpreted as an evolutionary tradeoff between fine-scale versus overall enamel distribution. On the one hand, unevenly thick enamel at a fine scale, combined with strongly decussated enamel as observed in P. pygmaeus, is expected to increase local resistance to crack propagation. On the other hand, uniformly thick enamel at the overall scale would improve the overall resilience of the enamel in coping with challenging food on a daily basis. Although understanding the effects of ecology on enamel distribution in apes requires further investigation, the results presented in this paper confirm the interest of enamel distribution for taxonomy and phylogeny.