Abstract

Present study was conducted to find out the effect of various botanical extract on the tissue, cellular an d sub cellular level and histopathology of silkworm, findings of the present study gives useful data concerning the changes in the insect. Three plant s extract viz Azadirachta indica, Ocimum sanctum and Parthenium hysterophorus were used as experimental while untreated leaves consider as control. These botanicals were sprayed on the tukra (Pink mealy bug) infected mulberry leaves and feed to silkworm (CSR2 bivoltine hybrid). Findings of the study suggested no change in the fat body of the silkworm feed on the botanical sprayed leaves and it was with normal vacuolization cytoplasm of cells. While hypertrophied nucleus fat body and voculated cytoplasm was reported in the silkworm fed on the tukra infected chawki leaves. The outer layers of the nucleolus were reported somewhat hypertrophied and cytoplasm was reported vacuolate with mild degeneration of cell in silkworm fed on the tukra infected leaves. Silk worm fed leaves revealed almost similar changes to that of normal and there was no change in botanical sprayed fed larvae. The impact in tissue of the silkworm when fed with normal and crude botanical extracts against mealy bugs shows normalcy, but in the t ukra infected mulberry leaves fed by silk worms the tissues sho ws slight degenerative with nutritional impact upon them. Mude Jagadish Naik* and Angothu Samba Naik

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