Abstract
EN
Highlights
In vitro methods are widely used in biomedical research and industry, often reducing or replacing animal experiments
A promising alternative for culture of human cells is the substitution of fetal bovine serum (FBS) with converted human AB serum (HABS), which is routinely tested for viral contamination
We tested this using colorectal (HT29), cervical (HeLa-229), and breast (MCF 7) cancer cell lines routinely used in cancer research, as well as primary human umbilical vein endothelial cells (HUVEC) and human dermal microvascular endothelial cells (HuDMEC)
Summary
In vitro methods are widely used in biomedical research and industry, often reducing or replacing animal experiments. A promising alternative for culture of human cells is the substitution of FBS with converted human AB serum (HABS), which is routinely tested for viral contamination. These studies suggest that HABS may be a good candidate to replace FBS as a supplement for cell culture.
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