Emulsion stabilized with phytoglycogen octenyl succinate prolongs the antimicrobial efficacy of ε-poly-l-lysine against Escherichia coli O157:H7

Abstract

ɛ-Poly-l-lysine (EPL) is a food-grade cationic antimicrobial compound with a wide antimicrobial spectrum against bacteria, yeasts, and molds. However, EPL can be subject to rapid depletion after initial application and lose activity quickly. To address this problem, this study used Escherichia coli O157:H7 and tryptic soy agar (TSA) deep-well depletion model to evaluate the prolonged antibacterial efficacy of EPL stabilized with emulsions formed by three types of emulsifier: (1) phytoglycogen octenyl succinate (PG-OS), an amphiphilic carbohydrate particulate; (2) waxy corn starch octenyl succinate (WCS-OS), an amphiphilic hyperbranched polysaccharide; and (3) Tween 20, a neutral small-molecule surfactant. During 20 days of storage at 4 °C, the residual antibacterial efficacy of EPL in PG-OS emulsion was the greatest. In contrast, Tween-20 and WCS-OS emulsions were not as effective as PG-OS emulsion to retain antibacterial activity. Meanwhile, equilibrium dialysis showed the greatest EPL retention with PG-OS emulsion, suggesting the impact of electrostatic and structural properties of emulsifiers at the oil-water interface on prolonged EPL efficacy against E. coli O157:H7.