Abstract
The seeds of two-row barley (Hordeum vulgare L.) cultivar ZJU3 were treated by chemical mutagen ethyl methane sulfonate (EMS) to develop a mutant library. In M2 generation, biological and agronomic characters were examined, and the identified mutants were validated in M3 generation. Totally 97 phenotype mutants, involved in seedling habits, maturity, leaf morphology, plant height, spike shape and spikelet fertility were obtained from 3 000 treated plants, with mutated frequency of 7.46%. The results indicate the feasibility of developing barley mutant population using EMS treatment and the discussion is done on forward genetics approach of gene function study using mutant population for barley genetic improvement.
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