Abstract
Induced mutagenesis offers a useful method to improve desirable characters in sugarcane (Saccharum spp. hybrids). In this study, eleven Ethyl Methyl Sulfonate (EMS)-induced mutants of the Indian sugarcane cultivar CoC 671 were evaluated for smut disease resistance along with agronomic and quality traits viz. early maturity, high sucrose, high cane yield and for quality. PCR-based early detection assay for smut resistance was successfully adopted using the Internal Transcribed Spacer sequence of Sporisorium scitamineum and the same was correlated with field-grown mutants exhibiting resistance upon artificial smut pathogen inoculation. The smut resistant mutants were also found superior for juice and sugar quality parameters such as Brix %, Sucrose %, Purity % as well as Commercial Cane Sugar content at 10th and 12th month maturity. Mutants TC 2819 and TC 2826 exhibited superior for sucrose content (20–24%) than parent CoC 671 (18–21%), respectively, at 10th and 12th month of maturity. The study highlights potential of EMS-induced genetic variability for the mutants with smut resistance and agronomic traits in sugarcane.
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