Abstract
The current study was conducted with aim to find an efficient simple protocol for propagation of stevia plant ( Stevia rebaudiana ) In Vitro. Shoot tips, leaves and stem nodes explants were sterilized with different concentrations of NaOCl (0.0, 2.0, 4.0 and 6% v/v) then cultured on MS medium supplemented with 30 g.l-1 sucrose. Results revealed that, no contamination was recorded when using 4.0 % and 6.0 % of NaOCl with (100% and 8.9%) survival at 4.0% and 6.0 % respectively. Moreover, combination of plant growth regulators which were used in this study, have been successfully optimized. However, best combination for number of shoots. explant-1 was found to be (0.4 BAP + 0.2 Kin mg.l-1) for shoot tips explants with an average of 16.44 while (2.0 BAP + 0.25 GA3 mg. l-1) produced maximum average of plant height from shoot tips reached 4.85 cm. For in vitro roots induction, maximum root induction, number and length of roots. Shootlets reached (93.3 %, 9.77 and 9.0 cm) respectively when cultured shootlets on MS medium supplemented with 2.0 mg.l-1 of IBA.
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