Employing an ICT-ESIPT strategy for ratiometric tracking of HClO based on sulfide oxidation reaction.

Abstract

Hypochlorous acid (HOCl) acts as crucial roles in pathologica processes and relevant diseases. Thus, it is meaningful to explore a reliable method for monitoring HClO in biosystem. In this work, a ratiometric fluorescent probe 2-(benzo[d]thiazol-2-yl)-4-(methylthio)phenol (BTMSP) has been constructed for HClO by adopting ICT-ESIPT strategy. The probe possessed itself red fluorescence due to the electron-donating capability of sulfur atom and showed remarkable blue fluorescence response to HClO by oxidizes the sulfur atom to a sulfoxide. The ratiometric probe exhibited highly specific, rapid response and excellent sensitivity toward HClO as well as a low detection limit (4.2×10-7M). Moreover, the ratiometric probe showed well-separated dual emission (450/580), and a large pseudo Stokes shift (190nm). In addition, the probe was used for the imaging of HClO with satisfying results.