Emphasis on the incorporation of Tropaeolin OO dye and silver nanoparticles for voltammetric estimation of flibanserin in bulk form, tablets and human plasma

Abstract

A novel electrochemical sensor based on the electro-deposition of silver nanoparticles (AgNPs) on Tropaeolin OO (poly-TO) layers over pencil graphite electrode (PGE) surface was fabricated for the first time for voltammetric determination of flibanserin (FBS); a drug enhances female sexual performance. Further characterization studies using cyclic voltammetry (CV), square wave voltammetry (SWV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM) were conducted. The AgNPs synergistic effect on poly-TO layers facilitates the FBS electro-oxidation in phosphate buffer solution (pH 6.0) and its determination in bulk form, tablets and in human plasma. Following ICH guidelines, validation of the proposed SWV method for FBS analysis was successfully achieved using the fabricated sensor (AgNPs@poly-TO/PGE). Under the optimal instrumental and experimental conditions, the anodic oxidation peak current was directly proportional to FBS concentration in the range from 0.1 to 8.5 μmol L−1 with low detection and quantitation limits (0.0286 and 0.0867 μmol L−1, respectively). High sensitivity, selectivity as well as easiness of fabrication are the main advantages of the modified sensor.