EMF Exposure Facilitates Wound Healing Events Modulating Cytokines Expression

Abstract

Event Abstract Back to Event EMF Exposure Facilitates Wound Healing Events Modulating Cytokines Expression Erica Costantini1*, Giovina Vianale1*, Chiara D'Angelo1*, Raffaella Muraro1* and Marcella Reale1* 1 Università G.D'Annunzio Chieti-Pescara, Italy Background: During wound repair inflammatory cytokines are upregulated sequentially leading to the activation of keratinocyte growth and migration. IL-1, secreted by the injured keratinocytes, drives both keratinocyte activation and production of TNFα that maintain keratinocytes in their activated state. Activated keratinocytes are hyperproliferative and migratory. Previous studies showed that extremely low frequency electromagnetic fields (ELF-EMF) are able to regulate cytokine expression. Objectives: On this basis the present study aimed to investigate the potential therapeutic use of ELF-EMF, evaluating, in HaCaT cells, the expression of cytokines that orchestrates successful wound. Methods: HaCaT cells were starved for 24 h in serum-free, then wounded with pipette tip to create a cell-free zone. HaCaT cells were exposed at 1 mT, 50Hz and compared to unexposed control cells. Wound healing was evaluated by measuring surface area, percentage and duration of healing. Cytokines expression was evaluated by RT-PCR in EMF-exposed and non-exposed HaCaT cells. Results: After 24 hours the scratch area covered by keratinocyte was increased in EMF-exposed compared to non-exposed samples, in accord with the observed cell proliferation induced by EMF. Moreover, in EMF-exposed HaCaT expression of IL-1, TNFa, IL-18 and MMP-2 was increased and IL-10, IL-18BP and MMP-9 was decreased. Conclusion: The ELF-EMF-enhanced expression of cytokines responsible for HaCaT proliferation and activation suggest an increased capacity of keratinocytes to reepithelialize the margin of wound avoiding the non-healing wounds. The ability of ELF-EMF to modulate cytokines expression highlights the potential therapeutic role of ELF-EMF in wound healing processes. Keywords: Wound Healing, keratinocyte, fibroblast, electromagnetic field, proliferation, Migration, RT-PCR Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Costantini E, Vianale G, D'Angelo C, Muraro R and Reale M (2013). EMF Exposure Facilitates Wound Healing Events Modulating Cytokines Expression. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00390 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Erica Costantini, Università G.D'Annunzio Chieti-Pescara, Chieti, Italy, erica.costantini@unich.it Dr. Giovina Vianale, Università G.D'Annunzio Chieti-Pescara, Chieti, Italy, gvianale@unich.it Dr. Chiara D'Angelo, Università G.D'Annunzio Chieti-Pescara, Chieti, Italy, chiara_dangelo@hotmail.it Prof. Raffaella Muraro, Università G.D'Annunzio Chieti-Pescara, Chieti, Italy, muraro@unich.it Prof. Marcella Reale, Università G.D'Annunzio Chieti-Pescara, Chieti, Italy, mreale@unich.it Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Erica Costantini Giovina Vianale Chiara D'Angelo Raffaella Muraro Marcella Reale Google Erica Costantini Giovina Vianale Chiara D'Angelo Raffaella Muraro Marcella Reale Google Scholar Erica Costantini Giovina Vianale Chiara D'Angelo Raffaella Muraro Marcella Reale PubMed Erica Costantini Giovina Vianale Chiara D'Angelo Raffaella Muraro Marcella Reale Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.