Abstract
There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic, and physiological similarities with humans. Due to recent advances in the sequencing of domestic animal genomes and the development of new organism cloning technologies, it is now very feasible to utilize pigs as a malleable species, with similar anatomic and physiological features with humans, in which to develop cancer models. In this review, we discuss genetic modification technologies successfully used to produce porcine biomedical models, in particular the Cre-loxP System as well as major advances and perspectives the CRISPR/Cas9 System. Recent advancements in porcine tumor modeling and genome editing will bring porcine models to the forefront of translational cancer research.
Highlights
Animal models have played a central role over the centuries in scientific investigations of human disease and treatment strategies
The KRAS-neo vector comprised: a short homology arm in KRAS intron 1; a transcriptional stop cassette comprising: a loxP site; adenoviral splice acceptor; promoterless neomycin phosphotransferase resistance gene; three poly-adenylation signals derived from SV40, bovine growth hormone and cytomegalovirus; and a second loxP site inserted into a ClaI site in KRAS intron 1; and a region of porcine KRAS extending from the ClaI site in intron 1 to a SacI site in intron 2, which included an engineered G to A point mutation within exon 2 that results in a glycine to aspartic acid substitution at codon 12 (G12D) (Li et al, 2015)
Both KRAS and TP53 transgenic pigs cells were transduced with 5 μM of Cre protein produced in vitro with the vector pTriExHTNC (Addgene plasmid 13763; Leuchs et al, 2012; Li et al, 2015)
Summary
Received: 03 November 2015 Accepted: 13 February 2016 Published: 29 February 2016. Citation: Schook LB, Rund L, Begnini KR, Remião MH, Seixas FK and Collares T (2016) Emerging Technologies to Create Inducible and Genetically. There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic, and physiological similarities with humans. Due to recent advances in the sequencing of domestic animal genomes and the development of new organism cloning technologies, it is very feasible to utilize pigs as a malleable species, with similar anatomic and physiological features with humans, in which to develop cancer models. We discuss genetic modification technologies successfully used to produce porcine biomedical models, in particular the Cre-loxP System as well as major advances and perspectives the CRISPR/Cas System. Recent advancements in porcine tumor modeling and genome editing will bring porcine models to the forefront of translational cancer research
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
