Abstract

Dieback of mango caused by Lasiodiplodia theobromae is among several diseases responsible for low crop production in Pakistan. To further complicate the issue, resistance in L. theobromae is emerging against different fungicides. L. theobromae was isolated from diseased samples of mango plants collected from various orchards in the Multan District. The efficacy of different fungicides viz. copper oxychloride, diethofencarb, pyrachlostrobin, carbendazim, difenoconazole, mancozeb, and thiophanate-methyl was evaluated in vitro using a poison food technique. Thiophanate-methyl at all concentrations was found to be the most effective among five systemic fungicides against L. theobromae, followed by carbendazim, difenoconazole and diethofencarb. The fungicides, i.e., thiophanate-methyl, difenoconazole, carbendazim and diethofencarb showed maximum efficacy with increasing concentration. The isolates of L. theobromae showed some resistance development against the tested fungicides when compared with previous work. These investigations provide new information about chemical selection for the control of holistic disease in mango growing zones of Pakistan.

Highlights

  • Mango (Mangifera indica L.) is one of the most important fruit in Punjab and Sindh Provinces of Pakistan with an average yield of 11.20 tons hectare (Anonymous, 2011)

  • 1 500 parts (3-4 mm) of diseased tissues from the collected samples were excised, disinfected in 1% sodium hypochlorite solution and washed twice in sterilized distilled water. They were dried on blotting paper and placed on sterilized Petri dishes lined with potato dextrose agar (PDA) (Bio Basic Inc.) at 25°C with illumination of 600 Lux (Ploetz and Gregory, 1993)

  • The infectious fungus L. theobromae was isolated from leaves and twigs/branches, and different fungicides were evaluated for its control

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Summary

Introduction

Mango (Mangifera indica L.) is one of the most important fruit in Punjab and Sindh Provinces of Pakistan with an average yield of 11.20 tons hectare (Anonymous, 2011). Various factors including diseases and insects are responsible for its low productivity (Shahbaz et al, 2009). Malik et al (2005) described the enigma of dieback as a serious disease for mango growers. The onset of dieback becomes clear by discoloration and darkening of twigs, oozing of gum, wilting of leaves, dieback, browning of vascular bundles and death of the entire plant (Narasimhudu and Reddy, 1992; Khanzada et al, 2004a). Previous research has proved Lasiodiplodia theobromae (Pat.) Griff. Botryodiplodia theobromae Pat.) as the cause of dieback of mango (Sutton, 1980; Khanzada et al, 2004b)

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