Emerging Porcine adenovirus PAdV-SVN1 and other enteric viruses in samples of industrialized meat by-products

Fernanda Gil De Souza,Artur Fogaça Lima,Victória Brandalise,Paula Rodrigues De Almeida,Andréia Henzel,Thalles Guillem Machado,Micheli Filippi,Viviane Girardi,Caroline Rigotto,Fernando Rosado Spilki

doi:10.1590/0103-8478cr20180931

Fernanda Gil De Souza, Artur Fogaça Lima + Show 8 more

Open Access

https://doi.org/10.1590/0103-8478cr20180931

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Journal: Ciência Rural	Publication Date: Jan 1, 2020
License type: CC BY 4.0

Affiliation: Universidade Feevale

Abstract

ABSTRACT: Foodborne diseases are often related to consumption of contaminated food or water. Viral agents are important sources of contamination and frequently reported in food of animal origin. The goal of this study was to detect emerging enteric viruses in samples of industrialized foods of animal origin collected in establishments from southern of Brazil. In the analyzed samples, no Hepatitis E virus (HEV) genome was detected. However, 21.8% (21/96) of the samples were positive for Rotavirus (RVA) and 61.4% (59/96) for Adenovirus (AdV), including Human adenovirus-C (HAdV-C), Porcine adenovirus-3 (PAdV-3) and new type of porcine adenovirus PAdV-SVN1. In the present research, PAdV-SVN1 was detected in foods for the first time. The presence of these viruses may be related to poor hygiene in sites of food preparation, production or during handling.

Highlights

Cases of foodborne diseases of diverse etiologies are often related to the consumption of food improperly handled, transported or badly stored (RODRIGUES et al, 2004)
Human adenoviruses (HAdV) are important etiological agents associated with urinary tract infections, respiratory diseases, conjunctivitis, gastroenteritis in both adults and children, and in immunocompromised patients it has been related to systemic fatal infections
Through sequencing and phylogenetic analysis of AdV (Figure 1) positive samples revealed three samples belonging to the Human adenovirus-C (HAdV-C), one sample belonging to the species Porcine adenovirus-3 (PAdV-3) and in 27 samples the emerging species PadV-SVN1 has been reported

Summary

Introduction

Cases of foodborne diseases of diverse etiologies are often related to the consumption of food improperly handled, transported or badly stored (RODRIGUES et al, 2004) Some enteric viruses such as Adenovirus (AdV), Rotavirus (RV) and Hepatitis E virus (HEV) are important causes of diseases for human beings, all transmitted by water and contaminated food (HELDT et al, 2016; SEITZ et al, 2011; SINCLAIR et al, 2009). Since there is no specific legislation establishing acceptable detection limits for foodborne viruses, their presence already renders it unfit for consumption (RODRIGUES et al, 2004) Among these pathogens, Human adenoviruses (HAdV) are important etiological agents associated with urinary tract infections, respiratory diseases, conjunctivitis, gastroenteritis in both adults and children, and in immunocompromised patients it has been related to systemic fatal infections The HAdV are non-enveloped viruses, with icosahedral capsisd and a doublestranded DNA genome; belonging to the Adenoviridae family, that includes the genus Mastadenovirus which infects mammals such as humans, swine, apes, cattle, horses, canines, sheep and rodents, being transmitted by oral fecal route (FONG & LIPP, 2005)

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