Year-end Sale % OFF 
Abstract
Multiciliated cells (MCCs) have several hair-like structures called cilia, which are required to propel substances on their surface. A cilium is organized from a basal body which resembles a hollow microtubule structure called a centriole. In terminally differentiated MCCs, hundreds of new basal bodies/centrioles are formed via two parallel pathways: the centriole- and deuterosome-dependent pathways. The deuterosome-dependent pathway is also referred to as “de novo” because unlike the centriole-dependent pathway which requires pre-existing centrioles, in the de novo pathway multiple new centrioles are organized around non-microtubule structures called deuterosomes. In the last five years, some deuterosome-specific markers have been identified and concurrent advancements in the super-resolution techniques have significantly contributed to gaining insights about the major stages of centriole amplification during ciliogenesis. Altogether, a new picture is emerging which also challenges the previous notion that deuterosome pathway is de novo. This review is primarily focused on studies that have contributed towards the better understanding of deuterosome-dependent centriole amplification and presents a developing model about the major stages identified during this process.
Highlights
Centrosomes act as major microtubule organizing centers in the majority of animal cells, forming a bipolar spindle during cell division
The authors reported that Deup1 protein levels are 7.3 fold higher as compared to CEP63 at day 3 of air–liquid interface (ALI), which indicates a dominant role of the DD pathway in centriole production during ciliogenesis
Zhao et al used 3D structured illumination microscopy (3D-SIM) to describe the six stages of centriole amplification in mouse tracheal epithelia cells (MTECs) at day 3 of ALI (Figure 2a). They observed that centriole amplification starts from stage II, where Deup1 foci was localized inside the ring of CEP152 and this ring was found to be decorated with several centrin-positive dots
Summary
Centrosomes act as major microtubule organizing centers in the majority of animal cells, forming a bipolar spindle during cell division. Laser ablation of pre-existing centrioles in somatic cells results into the generation of new centrioles via the de novo centriole pathway. In these cells, the de novo centriole organization was found to be significantly slow as compared to the template pathway [21,22]. Some deuterosome-specific proteins like Deup1 [25], CCDC78 [26] and CCNO [27] have been identified in the last five years, suggesting that the DD pathway is quite regulated These growing evidence mostly acquired through super-resolution microscopy and live imaging in MCCs instigates an interesting correlation between pre-existing centrioles and deuterosome organization [28]. We are at a juncture where a new picture of DD pathway is shaping up and, the present review summarizes all these studies which have contributed to the changing picture of the DD pathway in MCCs
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
