Abstract

The detection of cyanotoxins in aquatic environment is paramount due to its negative impact on aquatic biota. Various types of analytical techniques have been employed to qualify and quantify the presences of cyanotoxins in aquatic environment but most of these methods are not easily accessible. This mini-review concisely summarized the emerging approaches used to uncover cyanotoxins in aquatic environment. The methods of quantifying cyanotoxins in aquatic environment including bio-analytical methods, molecular methods and bioassay methods was compare with the efficiency of the use of analytical methods in the quantification of cyanotoxins from aquatic environment. The mini-review shows that although, analytical methods are highly effective in the determination of cyanotoxins in aquatic environment, these require a high-level laboratory skills and expertise whereas bio-analytical methods, molecular methods and bioassay method are highly sensitive, easily accessible and effective in the quantification of cyanotoxins in aquatic environment. These emerging techniques are important tools that can be used to prevent the toxicity associated with algae blooms because these methods have the capacity to detect the presences of micro quantity of these toxins in aquatic environment before algae blooms occur. These mini-review shows that bio-analytical methods, molecular methods and bioassay have limitations that make analytical methods the best option for the detection of cyanotoxins in aquatic environment despite the fact that high skills are needed to actualized the aim, it is thereby recommended that more research should be conducted to eradicate the challenges associated with the use of biological methods for the detection of cyanotoxins in aquatic environment.

Highlights

  • The algal blooms produced by cyanobacteria have shading effect on the lower layers, resulting in more respiratory activities below the surface and suffocation of fauna 1,2

  • Determination of Cyanotoxins using Analytical Methods The most reliable methods of detecting cyanotoxins in aquatic environment is by the usage of high enactment liquid chromatography (HPLC), this method permits the characterization of the toxins into toxins kinds and variants.[46]

  • The methods of quantifying cyanotoxins in aquatic environment including bio-analytical methods, molecular methods and bioassay methods were compared with the efficiency of the use of analytical methods in the quantification of cyanotoxins from aquatic environment

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Summary

Introduction

The algal blooms produced by cyanobacteria have shading effect on the lower layers, resulting in more respiratory activities below the surface and suffocation of fauna 1,2. Determination of Cyanotoxins using Analytical Methods The most reliable methods of detecting cyanotoxins in aquatic environment is by the usage of high enactment liquid chromatography (HPLC), this method permits the characterization of the toxins into toxins kinds and variants.[46] The limitation of the HPLC method in cyanotoxin detection is the fact that it is expensive and difficult to access.[47] The concentration of cyanotoxin in the tissues of animals is usually determine using the high-performance liquid chromatographic (HPLC, LC) technique attached with UV, photodiode array (PDA) and/or mass spectrometer (MS) detectors was applied.[48] These process permits further detailed identification according to retention time but possess further limitations which include prolonged examination time resulting from purification, concentration and scarcity of commercially cyanotoxins standards.[49] This method is not the best method for the quick determination of cyanotoxin with low concentration and continuous sample investigation. This multi-format requisited less than 4 h to complete sample collection and up to 100 dissimilar species can be identified simultaneously in a single sample.[55]

Conclusion
Methods to detect cyanobacteria and their
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