Abstract
BackgroundA study conducted at the International Airport of Cluj-Napoca, Romania, with the aim of investigating the presence/absence of invasive Aedes mosquito species resulted in finding Aedes japonicus japonicus (Theobald 1901) eggs in one of the ovitraps placed on site.MethodsThe study was carried out between 30 June and 29 September 2020. On 24 August, 26 eggs were collected and later hatched at the University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca’s insectary. On 15 October another adult female Ae. japonicus was caught entering a building in the center of the city, about 7 km from the first sampling spot.ResultsThe mosquitoes were identified morphologically and confirmed by molecular analysis, based on the genetic analysis of the mitochondrial gene cytochrome c oxidase subunit 1 (COI).ConclusionThis is the first report of the species in Romania, highlighting the need for surveillance and implemented control methods. However, in Romania to our knowledge only Aedes albopictus has been established; further studies are required to learn about this new invasive species' status in Romania.Graphical
Highlights
MethodsThe study was carried out between 30 June and 29 September 2020
A study conducted at the International Airport of Cluj-Napoca, Romania, with the aim of investigating the presence/absence of invasive Aedes mosquito species resulted in finding Aedes japonicus japonicus (Theobald 1901) eggs in one of the ovitraps placed on site
Study area and design An entomological survey was conducted for 3 months at the ‘Avram Iancu’ International Airport in Cluj-Napoca, Romania, to detect invasive Aedes species (Fig. 1)
Summary
Study area and design An entomological survey was conducted for 3 months (between 30 June and 29 September 2020) at the ‘Avram Iancu’ International Airport in Cluj-Napoca, Romania, to detect invasive Aedes species (Fig. 1). The eggs were later hatched at the University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca’s insectary by submerging the polystyrene pieces in tap water in a controlled environment, where the room temperature was set at 30 °C and the humidity at 55%. Larvae were fed with TetraMin Mini Granules (Tetra GmbH, Germany) Another female mosquito resembling an invasive species was accidentally caught inside a building in Cluj-Napoca’s central area, spontaneously and unrelated to the study design. The DNA was extracted individually from each female mosquito specimen using ISOLATE II Genomic DNA Kit (Bioline Meridian Bioscience, Luckenwalde Germany), according to the manufacturer’s instructions and stored at − 20 °C. PCR products were purified using the ISOLATE II PCR and Gel Kit (Bioline Meridian Bioscience, Luckenwalde Germany) and sent for sequencing (Macrogen Europe, Amsterdam, The Netherlands). Mapping The maps were generated using QGis 3.6.2 software (http://www.qgis.org)
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