EMERGENCE OF PLASMID MEDIATED COLISTIN RESISTANCE GENE MCR-1 IN CARBAPENEM-RESISTANT PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES FROM ALGERIA: A NEW SUCCESSFUL RESISTANCE COMBINATION TOWARD A THERAPEUTIC IMPASSE?

Abstract

Ponte Academic JournalApr 2020, Volume 76, Issue 4 EMERGENCE OF PLASMID MEDIATED COLISTIN RESISTANCE GENE MCR-1 IN CARBAPENEM-RESISTANT PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES FROM ALGERIA: A NEW SUCCESSFUL RESISTANCE COMBINATION TOWARD A THERAPEUTIC IMPASSE?Author(s): Addouda Abir ,Ayachi Ammar, Hassaine Hafida, Dhaouadi Sana, Soufi Leila, Mahjoubi Mouna, Benmehidi Messaoud, Grainat Nadia, Elandalousi Ramzi BoubakerJ. Ponte - Apr 2020 - Volume 76 - Issue 4 doi: 10.21506/j.ponte.2020.4.16 Abstract:Objectives: Pseudomonas aeruginosa is one of the main causes of nosocomial infections,\r\nespecially in immunocompromised patients in burn and intensive care units, who have\r\ninherited a number of drug resistance determinants via horizontal mutation. For this reason,\r\nour work has also been conducted to prove the genetic relationship between strains and to\r\ninvestigate the occurrence of genes conferring high levels of carbapenem and colistin\r\nresistance in Pseudomonas aeruginosa isolates from the Algerian hospital.\r\nMethodology: 27 colistin resistant Pseudomonas aeruginosa (CO-RPA) recovered from\r\npatients and hospital environment were analyzed using a combination of phenotypic and\r\nmolecular typing methods for the identification of plasmid mediated colistin resistance genes;\r\ncarbapenem resistant Pseudomonas aeruginosa (CRPA) isolates were tested for the presence\r\nof genes encoding β-lactamases, quinolones and β-lactamases.\r\nPCR-Ribotyping and PCR 16s rDNA were used to establish the phylogeny and clonal\r\nrelationship of strains.\r\nResults: Molecular Screening for mcr encoding genes showed that 02 tested isolates\r\nexamined contained mcr-1, one isolate of which expressed mcr-1 and OprD genes at the same\r\ntime. Interestingly, 62.95 % of the colistin-resistant strains were imipenem-resistant in this\r\nstudy. Actually, loss/mutation in OprD was founded in 29, 41% (05/17) of imipenem-resistant\r\nstrains. PCR- Ribotyping revealed an outbreak of clonal distribution of colistin and\r\ncarbapenem resistant Pseudomonas aeruginosa isolates.\r\nConclusion: The current study highlights the first potential spread of the mcr-1 gene in\r\nPseudomonas aeruginosa strains in the health care setting in Algeria and around the world\r\nthat appears to be linked to the uncontrolled use of antibiotics. Strict precautions will be\r\nexpected to prevent the further spread of these pathogens in hospital settings. Download full text:Check if you have access through your login credentials or your institution Username Password