Emergence of extended spectrum beta-lactamase (ESBLs) producing Proteus in raw milk of Doon Valley

Abstract

The present study deals with the determination of extended spectrum of ß-lactamase (ESBL) producing Proteus in raw milk of Doon Valley. ESBL producing isolates were screened by double disc approximation test using commercially available ß-lactam (Piperacillin) and ß-lactam/ß-lactamase inhibitor combination (Piperacillin/ Tazobactam). All isolates of Proteus sp. were reported to resistant against Methicillin and were sensitive to Piperacillin, Cephotaxime, Ceftazidime, and Cefoperazone. This indicates that all the isolates were able to produce ß-lactamase in low and higher amount. This amount of ß-lactamase is inactivated by Tazobactam (ß-lactamase inhibitor) and the zone of inhibition with Piperacillin/Tazobactam combination was greater as compared to Piperacillin alone. There was a significant difference (>4 mm) in zone of inhibition was reported with Piperacillin and in combination of Piperacillin and Tazobactam. Hence, the overall emergence of ß-lactamase producing Proteus sp. in raw milk of Dehradun city was 100%, which is an alarming situation for public health and needs serious concern.