Abstract

Environmental Microorganism Data Set Fifth Version (EMDS-5) is a microscopic image dataset including original Environmental Microorganism (EM) images and two sets of Ground Truth (GT) images. The GT image sets include a single-object GT image set and a multi-object GT image set. EMDS-5 has 21 types of EMs, each of which contains 20 original EM images, 20 single-object GT images and 20 multi-object GT images. EMDS-5 can realize to evaluate image preprocessing, image segmentation, feature extraction, image classification and image retrieval functions. In order to prove the effectiveness of EMDS-5, for each function, we select the most representative algorithms and price indicators for testing and evaluation. The image preprocessing functions contain two parts: image denoising and image edge detection. Image denoising uses nine kinds of filters to denoise 13 kinds of noises, respectively. In the aspect of edge detection, six edge detection operators are used to detect the edges of the images, and two evaluation indicators, peak-signal to noise ratio and mean structural similarity, are used for evaluation. Image segmentation includes single-object image segmentation and multi-object image segmentation. Six methods are used for single-object image segmentation, while k-means and U-net are used for multi-object segmentation. We extract nine features from the images in EMDS-5 and use the Support Vector Machine (SVM) classifier for testing. In terms of image classification, we select the VGG16 feature to test SVM, k-Nearest Neighbors, Random Forests. We test two types of retrieval approaches: texture feature retrieval and deep learning feature retrieval. We select the last layer of features of VGG16 network and ResNet50 network as feature vectors. We use mean average precision as the evaluation index for retrieval. EMDS-5 is available at the URL:https://github.com/NEUZihan/EMDS-5.git.

Highlights

  • 1.1 Environmental MicroorganismsAll the time, Environmental Microorganisms (EMs) [1] are part of our environment

  • We summarize the above noises and count them into 13 kinds of noise to add noise to the original image respectively, and use different filters for denoising

  • In order to prove the effectiveness of our EMDS-5 in edge detecition evaluation, seven operators are used to detect edges

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Summary

Environmental Microorganisms

Environmental Microorganisms (EMs) [1] are part of our environment. EMs bring us benefits, while others affect our physical health. Many researchers devote themselves to study these microorganisms to improve our lives. Image analysis has a great significance for the analysis of EM images. It can help researchers to analyze the types and forms of EMs. For example, Rotifera is a common EM and it is widely distributed in lakes, ponds, rivers and other brackish water bodies, having great significance in the study of ecosystem structure function and biological productivity because of their extremely fast reproduction rate and high yield. Arcella is a kind of common EMs. Arcella mainly feeds on plant giardia and single-celled algae. An oligoplastic water body is the most suitable living environment.

Application scenarios of Environmental Microorganisms
Contribution
Dataset information of EMDS-5
Evaluation of image denoising methods
Evaluation of edge detection methods
Single-object image segmentation
Multi-object image segmentation
Feature extraction evaluation using EMDS-5
Multi-object feature extraction
Image classification evaluation using EMDS-5
Image retrieval evaluation using EMDS-5
Texture feature based image retrieval using EMDS-5
Deep learning feature based image retrieval using EMDS-5
Conclusion and future work

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