Abstract
Pharmacognosy Research,2021,13,3,135-139.DOI:10.5530/pres.13.3.5Published:June 2021Type:Original ArticleAuthors:Thiago Felix da Silva, Maria Isabel de Assis Lima Castro, Marta Gerusa Soares de Lucena, Camilla Vila Nova Soares Silva, Laisla Rangel Peixoto, Eliane Alves Bandeira de Carvalho, Silvania Tavares Paz, Beathryz Lethycya Almeida Guimarães, José Maria Barbosa Filho, and Eliete Cavalcanti da Silva Author(s) affiliations:Thiago Felix da Silva1,*, Maria Isabel de Assis Lima Castro2, Marta Gerusa Soares de Lucena3, Camilla Vila Nova Soares Silva4, Laisla Rangel Peixoto5, Eliane Alves Bandeira de Carvalho6, Silvania Tavares Paz7, Beathryz Lethycya Almeida Guimarães3, José Maria Barbosa Filho8, Eliete Cavalcanti da Silva1 1Postgraduate Program in Morphotechnology - Federal University of Pernambuco, Recife, PE, BRAZIL. 2Master of Sciences; Postgraduate Program in Biological Sciences - Federal University of Pernambuco, Recife, PE, BRAZIL. 3Department of Histology and Embryology - Federal University of Pernambuco, Recife, PE, BRAZIL. 4Multiprofessional Residency Program in Health Surveillance - Recife Health Department, Recife, PE, BRAZIL. 5Master of Sciences; Institute of Drugs and Medicines Research - Federal University of Paraíba, João Pessoa, PB, BRAZIL. 6Master of Sciences; Postgraduate Program in Biological Sciences - Federal University of Pernambuco, Recife, PE, BRAZIL. 7Master of Sciences; Department of Pathology - Federal University of Pernambuco, Recife, PE, BRAZIL. 8Institute of Drugs and Medicines Research - Federal University of Paraíba, João Pessoa, PB, BRAZIL. Abstract:Introduction: Yangambin, a lignan predominant in the leaves of Ocotea duckei Vattimo-Gil, has several biological activities, such as anti-convulsant, analgesic, anti-inflammatory and leishmanicidal. Objectives: The aim of this study was to evaluate the embryotoxicity of yangambin on the neurodevelopment of Gallus gallus domesticus embryos. Materials and Methods: 120 fertilized eggs were divided in three groups: G1 (PBS with 0.1 % Tween 80), G2 (50 μg/ml yangambin) and G3 (65 μg/ml yangambin) and each egg was inoculated with 100 μL of the respective solutions. The fertilized eggs were incubated at a temperature of 37.5°C, with a relative humidity of 65% to 75%, for 48 hr and then their embryos were histologically processed. Results: In staging, carried out according to Hamburger and Hamilton (1951), variations of stages were identified. In all groups, the morphological analysis revealed the closure of the anterior neuropore and absence of malformations in the optic vesicles and in the secondary encephalic vesicles. In the caudal region, a standard development of the neural tube was observed, with well-segmented somites and regression of the primitive line. The cross sections showed that the internal structure of the somite’s, composed of dermatome, myotome and sclerotome, was preserved. The statistical analysis did not show significant differences between the groups regarding the morphometry of the cephalic and caudal regions of the neural tube. Conclusion: Yangambin did not show embryotoxic effects on the neurodevelopment of Gallus gallus domesticus embryos, under the tested conditions. Keywords:Chicken embryo, Lauraceae, Lignan, Neurodevelopment, Secondary metabolitesView:PDF (809.58 KB)
Highlights
Yangambin, a lignan predominant in the leaves of Ocotea duckei Vattimo-Gil, has several biological activities, such as anti-convulsant, analgesic, anti-inflammatory and leishmanicidal
Materials and Methods: 120 fertilized eggs were divided in three groups: G1 (PBS with 0.1 % Tween 80), G2 (50 μg/ml yangambin) and G3 (65 μg/ml yangambin) and each egg was inoculated with 100 μL of the respective solutions
The morphological analysis revealed the closure of the anterior neuropore and absence of malformations in the optic vesicles and in the secondary encephalic vesicles
Summary
A lignan predominant in the leaves of Ocotea duckei Vattimo-Gil, has several biological activities, such as anti-convulsant, analgesic, anti-inflammatory and leishmanicidal. Objectives: The aim of this study was to evaluate the embryotoxicity of yangambin on the neurodevelopment of Gallus gallus domesticus embryos. The statistical analysis did not show significant differences between the groups regarding the morphometry of the cephalic and caudal regions of the neural tube. Conclusion: Yangambin did not show embryotoxic effects on the neurodevelopment of Gallus gallus domesticus embryos, under the tested conditions. Lignans are present in Ocotea duckei Vattimo-Gil (Lauraceae), popularly known as “Louro-de-cheiro”, “Louro-pimenta” or “Louro-canela” and is widely distributed in the Northeast of Brazil, having in its leaves a furofuran lignan, called yangambin, which is the major constituent of the total fraction of lignoids of the species.[4,5,6]. The therapeutic potential of medicinal plants is related to natural products, which are sources of several bioactive molecules and when isolated, their biological activities can be investigated through experimental models.[14,15] it is necessary to include toxicity tests to identify the effects of the interaction between these compounds and the organism, in addition to ensuring that these substances do not cause harmful effects.[16]
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