Abstract
Chondrichthyans (cartilaginous fishes) exhibit highly variable reproductive styles, categorized as viviparity and oviparity. Among these, species with oviparity provide an enormous potential of molecular experimentation with stable sample supply which does not demand the sacrifices of live mothers. Cartilaginous fishes are divided into two subclasses, chimaeras (Holocephali) and elasmobranchs (Elasmobranchii), and the latter consists of two monophyletic groups, Batoidea (rays, skates and torpedoes) and Selachimorpha (sharks). Here we report transcriptome assemblies of the ocellate spot skate Okamejei kenojei, produced by strand-specific RNA-seq of its embryonic tissues. We obtained a total of 325 million illumina short reads from libraries prepared using four different tissue domains and assembled them all together. Our assembly result confirmed the species authenticity and high continuity of contig sequences. Also, assessment of its coverage of pre-selected one-to-one orthologs supported high diversity of transcripts in the assemblies. Our products are expected to provide a basis of comparative molecular studies encompassing other chondrichthyan species with emerging genomic and transcriptomic sequence information.
Highlights
Background & SummaryOviparous chondrichthyans are distributed into three different selachimorphan orders, Carcharhiniformes, Heterodontiformes, and Orectolobiformes, and one batoid order Rajiformes
One of the genera in Rajidae is Okamejei, and among more than a dozen of species in the genus Okamejei, we focused on the ocellate spot skate Okamejei kenojei in the present study
All animal experiments were conducted in accordance with the Guideline of the Institutional Animal Care and Use Committee (IACUC) of RIKEN Kobe Branch (Approval ID: H16-11)
Summary
Oviparous (egg-laying) chondrichthyans are distributed into three different selachimorphan (shark) orders, Carcharhiniformes, Heterodontiformes, and Orectolobiformes, and one batoid (skate) order Rajiformes (summarized in). As of February 2018, molecular sequence data for rajiforms concentrate on species in Rajidae, such as the little skate Leucoraja erinacea, whose genome is being sequenced, in the head of the list, and it is followed by Amblyraja radiata and Raja clavata. One of the genera in Rajidae is Okamejei, and among more than a dozen of species in the genus Okamejei, we focused on the ocellate spot skate Okamejei kenojei in the present study. This species was recognized as Raja porosa, Okamejei porosa, or Raja kenojei. The authors’ group proposed some of those factors[5], and in the present study, we extend those factors by incorporating latest reagents for sample preparation and tools for sequence data analysis
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