Abstract
Energy homeostasis is an essential process during oogenesis, nutrients are required for suitable embryonic development, and recently, studies have investigated metabolic activity during this process. This work aims the investigation of dynamics of energy source utilization of Rhynchophorus palmarum during embryogenesis. For this, we first evaluated the mobilization kinetics of the lipids and glycogen. Thereafter, the synthesis of RNA, protein, and the involvement of enzyme of the glycolytic and pentose-phosphate pathways. Results showed that lipid content decreased in contrast with the lipase activity. The total glycogen amounts it was partly consumed and the glucose content increased, but then values remained stable until hatching. Total RNA content increased, and no significant changes in total protein content were observed. A study of the glycolytic pathway data showed activity of hexokinase and pyruvate kinase at the beginning of embryogenesis. Furthermore, glucose-6-phosphate formed is driven into the pentose-phosphate pathway viewed the high activity of glucose-6-phosphate dehydrogenase. Finally, these results showed that mobilization of different energy sources together with different enzymatic activities has an important role in embryonic development of R. palmarum .
Highlights
Energy homeostasis is an essential process during oogenesis, nutrients are required for suitable embryonic development, and recently, studies have investigated metabolic activity during this process
Results showed that lipid content decreased during embryogenesis, dropping to 1.8 lg/egg at 24 h (P < 0.05) and 1.5 lg at 36 h (P < 0.01) (Fig. 1A)
This work evaluated the kinetics from the utilization of the main potential energy sources available during the embryonic development of the R. palmarum
Summary
Energy homeostasis is an essential process during oogenesis, nutrients are required for suitable embryonic development, and recently, studies have investigated metabolic activity during this process. Glucose-6-phosphate formed is driven into the pentose-phosphate pathway viewed the high activity of glucose-6-phosphate dehydrogenase These results showed that mobilization of different energy sources together with different enzymatic activities has an important role in embryonic development of R. palmarum. Sanchez et al (1993) studied the biology and the behavior of the insect including courtship, mating, and oviposition in the laboratory They indicated that the females deposit their eggs into holes in the plant made by the rostrum, normally when the surface of the plant tissue presents some damage, near or on the internodal area of the palm trunk. The Tribolium castaneum’s metabolic status during early embryogenesis showed that glucose and glycogen regulation is important for early T. castaneum embryonic patterning (Fraga et al 2013) Given these facts, it is clear that the embryos development has different mechanisms for use of substrates, such as amino acids, lipids, and glucose to control the flow of energy in embryogenesis. Hexokinase, pyruvate kinase, and glucose-6-phosphate dehydrogenase suggests important roles of these enzymes during embryogenesis
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