Embryonic and postembryonic development of serial homologous neurons in the subesophageal ganglion of Tenebrio molitor (Insecta: Coleoptera).

Abstract

Neuroblast pattern, engrailed expression and proliferation in the subesophageal neuromers of the beetle Tenebrio molitor are characterized throughout embryogenesis. The proliferation of neuroblasts has been studied throughout postembyronic development. Serotonin, crustacean cardioactive peptide and tyrosine hydroxylase-like-immunoreactive neurons are characterized and their neuronal development has been studied. There is an initial posterior-anterior gradient in neuroblast segregation leading to a reduced number of neuroblasts in the frontal subesophageal neuromer. The study of the engrailed expression shows that only the anterior subfraction of the neuromeral neuroblast configuration is reduced, whereas the posterior two rows of engrailed-positive neuroblasts are not affected during the first 40% of embryogenesis. The overall number of proliferations in the first subesophageal neuromer reaches only 30-50% of the value found in each of the other two neuromers. The analysis of serotonin and crustacean cardioactive peptide immunoreactivity allows the identification of serial homologous neurons which persist from the early embryo to the adult stage. In the different gnathal neuromers, these neurons form structurally highly similar projection patterns, but show different extensions of their arborizations, corresponding to the relative size of each neuromer. Structural homologies between subesophageal and thoracic neuromers are discussed.