Abstract

Iris sangumea Dorm ex Horn. is a perennial cold-tolerant herbs of the Iris genus of Iridaceae, which has a well-formed, bright color and adaptability. Through understanding the flower bud differentiation, Sporogenesis, gametogenesis, fertilization and embryogenesis of I. sangumea, we developed flowering regulation and control breeding. I. sangumea flower bud differentiation was observed in April for 21 days; in this stage, the early differentiation process was slow, temperature was more than 5 °C, if the temperature is shorter than this, most flower bud will have no differentiation, and turn to vegetative growth; later differentiation is faster and required temperature is from 5 to 8 °C and gradually rose to 10‒12 °C. The temperature in April plays the definitely role in the number of bud differentiation. Three stamina contain tetrasporangiate anthers and a glandular tapetum. The anther wall is composed of four cell layers inclduding the epidermis, the endothecium, one middle layer and the secretory tapetum. The type of anther wall development the dicotyledonous type. After pollen maturation, the anther wall forms a double-layer containing only epidermis and endothecium. Simultaneous cytokinesis is of the continuous type during meiosis of microspore mother cell results in a zygomorphous or tetragonal tetrad. Mature pollen grains are two-celled. I. sangumea’s stylar canal is hollow, open type style, and the gynoecium has three carpels. The ovaries have axial placentas. The ovule is anatropous, bitegmic and crassinucellate. The primary sporogenous cell differentiates directly as the megaspore. The megaspore tetrads are linear, T-shaped or juxtaposed. Generally, the first of the tetrad megaspores at the chalaza becomes functional while the other three degenerate. A mature embryo sac with seven cells and eight nuclei embryo sac is of the polygonum type. For dichogamy, the male gametophyte matures about 2 days earlier than the female gametophyte. The pollen tube entered into the embryo sac for 22–24 h after pollination. The dormancy stage of fertilized ovules is 5‒6 days, and the fusion of sperm and egg nucleus is of the premitotic type. The primary endosperm cell has shorter dormancy of 4‒6 days, and endosperm formation is of the nuclear type.

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