Embryogenesis in Valerian (Valeriana officinalis L.) using leaf segments

Abstract

Abstract: A simple procedure for direct embryogenesis without an intervening callus production was developed in valerian (Valeriana officinalis L.) using the leaf segments. Direct somatic embryogenesis were induced using by half-strength MS medium supplemented with 2,4-D (0.5 mg·l -1 ), glutamic acid (100 mg·l -1 ), 4% sucrose and 8 g·l -1 agar. Germinated embryo to form plantlets enhanced on MS medium supplemented with NAA (0.1 mg·l -1 ) and Kin (2 mg·l -1 ). Regenerated plants with well-developed root and shoot systems were successfully (72%) transferred to greenhouse.