Abstract
AbstractEmbryogenic callus was induced by culturing explants of pumpkin hypocotyls on Murashige‐Skoog‐medium with the addition of 3% glucose and one of the following growth substances (or combinations of them): β‐indolylbutyric acid, 2,4‐dichlorophenoxyacetic acid, β‐indolylacetic acid, α‐naphthyl‐acetic acid, adenine (natural), kinetin, autoclaved water‐melon sap and yeast extract (Difco). A large number of embryoids and adventive buds were produced. These were able to develop to normal plants.The 17 strains of embryogenic tissue obtained have maintained their embryogenic characteristics for more than 3 years. The induction of embryogenic callus in pumpkin seems to be strongly dependent on the genetic constitution of each individual plant.
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