Elution of human satellite DNAs on a methylated albumin kieselguhr chromatographic column:Isolation of satellite DNA IV

Abstract

Human DNA has been eluted on a methylated albumin kieselguhr column: the fractions obtained have been centrifuged in Ag +-Cs 2SO 4 to show the presence of satellite DNAs. Satellite DNA II (density in CsCl = 1.693 g/ml) and the new satellite DNA IV (density in CsCl = 1.700 g/ml) are eluted early from the column and band, respectively, on the heavy side and the light side compared to the main DNA band in Ag +-Cs 2SO 4. Satellite DNA I (density in CsCl = 1.687 g/ml) and III (density in CsCl = 1.696 g/ml) are eluted late from the column. Satellite DNA IV separates into the complementary strands displaying a difference in density of 0.012 g/ml in alkaline CsCl and renatures rapidly after heat denaturation.