Elucidation of seed dormancy and phytohormones by germination after exogenous foliage whey application

Jadhav Rajesh K, Pranay Mulam, Khot Vidhesh

doi:10.25081/jpsp.2019.v5.3766

Abstract

A study was conducted with an aim of achieving 100% seed germination and to evaluate the rate of dormancy upon exogenous application of the deproteinised leaf juice (DPJ) in various leguminous and non leguminous seeds. DPJ if inducing retardation of dormancy, the activity of enzyme was analysed to prove the presence of phytohormones. DPJ from the three non leguminous foliages of brinjal, radddish and dasheen were prepared by green crop fractionation. The seed germination results obtained by the paper towel method and the activity of enzyme protease in DPJ of radish and Colocasia leaves separately. There was variation in germination rate by different DPJ influence. Some seeds showed fast germination while some germinated late. Few seeds were not germinated by control. In some seeds, DPJ decreased the rate of germination while on the contrary there was rapid growth of seedlings by the potentiality of DPJ. DPJ found mutagenic by its influencing inhibitory expression in seedling growth in some cases. The enzyme protease released by phytohormone gibberellin during the seed germination. Positive glyoxlic test indicated presence of auxins in whey.

Highlights

The yields of extractable protein from leaves taken at the time of harvest of the edible part from brassicas, radish ranged between 76 to 170 kg/ha [1, 2]
To overcome the dormancy and to initiate rapid germination, the treatment of deproteinised juice (DPJ) prepared from Colocasia and radish given to crops, showed efficacy in optimization in the rate of germination by breaking the dormancy in specific crops viz., horsegram and ragi
While the DPJ from Raphanus was found suitable for the rapid germination of sunflower seeds

Summary

Introduction

The yields of extractable protein from leaves taken at the time of harvest of the edible part (root) from brassicas, radish ranged between 76 to 170 kg/ha [1, 2]. In Leaf protein research [4], the green crop is fractionated to pulp and squeezed. The juice and pressed crop are the byproducts of GCF (Green crop fractionation). The liquid residue obtained by juice filtration called as deproteinised juice (DPJ) is another by-product. The enzymes present in it are denatured after heating at 90°C. During this process, the DPJ is thrown randomly. When the juice is heated, majority of the nutrients are disposed in the deproteinised juice including enzymes and hormones. Few amino acids retains in DPJ during juice filtration

Methods

Results

Conclusion