Abstract
Gonadotropin-releasing hormone receptor (GnRHR) gene plays an important role in endocrine regulation as well as gonadal development. Two SNPs (G286A and T340C) of GnRHR were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and 3 combinations of these SNPs were observed. The single SNP and their genetic effects on semen quality traits were evaluated and a significant association with semen volume and concentration was found in T340C SNP. The mean semen volume and concentration of individuals with genotype TT showed significantly higher sperm concentration and lower semen volume compared to bulls with heterozygote TC genotype. The results of combined genotypes analysis of two SNPs showed that GGTC had the significantly highest semen volume and lowest concentration compare to the others.
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