Elucidating the hypoxic stress response in barley (Hordeum vulgare L.) during waterlogging: A proteomics approach

Haiye Luan,Chao Lv,Rugen Xu,Huiquan Shen,Baojian Guo,Yuhan Pan

doi:10.1038/s41598-018-27726-1

Abstract

Waterlogging is one of the major abiotic stresses that affects barley production and yield quality. Proteomics techniques have been widely utilized to explore the mechanisms involved in the responses to abiotic stress. In this study, two barley genotypes with contrasting responses to waterlogging stress were analyzed with proteomic technology. The waterlogging treatment caused a greater reduction in biomass and photosynthetic performance in the waterlogging-sensitive genotype TF57 than that in the waterlogging-tolerant genotype TF58. Under waterlogging stress, 30, 30, 20 and 20 differentially expressed proteins were identified through tandem mass spectrometry analysis in the leaves, adventitious roots, nodal roots and seminal roots, respectively. Among these proteins, photosynthesis-, metabolism- and energy-related proteins were differentially expressed in the leaves, with oxygen-evolving enhancer protein 1, ATP synthase subunit and heat shock protein 70 being up-regulated in TF58. Pyruvate decarboxylase (PDC), 1-amino cyclopropane 1-carboxylic acid oxidase (ACO), glutamine synthetase (GS), glutathione S-transferases (GST) and beta-1, 3-glucanase in adventitious, nodal and seminal roots were more abundant in TF58 than those in TF57 under waterlogging stress. Ten representative genes were selected for validation by qRT-PCR in different genotypes with known waterlogging tolerance, and the expression levels of three candidate genes (PDC, ACO and GST) increased in the roots of all genotypes in response to the waterlogging stress. These three genes might play a significant role in the adaptation process of barley under waterlogging stress. The current results partially determined the mechanisms of waterlogging tolerance and provided valuable information for the breeding of barley with enhanced tolerance to waterlogging.

Highlights

Waterlogging is one of the major abiotic stresses that affects barley production and yield quality
The plant height, tillers, leaf area, shoot fresh weight and dry weight of TF57 decreased by 40%, 26%, 50%, 59%, and 42%, respectively, while the same parameters for TF58 were reduced by only 13%, 3%, 17%, 16%, and 4%, respectively, compared to those of the control (Fig. 2)
The soil-plant analysis development (SPAD) value of TF57 decreased by 39.2% relative to the control, whereas that of TF58 was reduced by only 11.7% (Fig. 2)

Summary

Introduction

Waterlogging is one of the major abiotic stresses that affects barley production and yield quality. Ten representative genes were selected for validation by qRT-PCR in different genotypes with known waterlogging tolerance, and the expression levels of three candidate genes (PDC, ACO and GST) increased in the roots of all genotypes in response to the waterlogging stress. These three genes might play a significant role in the adaptation process of barley under waterlogging stress. Proteomic technique coupled with mass spectrometry (MS) can detect translational and post-translational regulations of different proteins These techniques have been widely applied in barley to explore the mechanisms involved in their response to abiotic stress, including salt, drought and low O29–11. Five other genotypes with known waterlogging tolerance were used to validate the results

Methods

Results

Conclusion