Elucidating resistance in cotton genotypes to whitefly, Bemisia tabaci, by population buildup studies

Abstract

Plant resistance has become an important component of integrated pest management (IPM) for management of whitefly, Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae), an important pest of cotton in India. The present studies were undertaken to standardize the plant stage and identify resistant cotton genotypes against whitefly. Nine plant stages of F846, a susceptible cotton genotype, were exposed to whitefly for 25 days under no-choice conditions. The population buildup (eggs, nymphs, pupae and adults) was recorded. The 12-, 14- and 16-leaf stages were suitable for plant resistance studies against whitefly, and the 14-leaf stage was taken for further studies. Ten cotton genotypes of Gossypium hirsutum and two of G. arboreum were covered with split cages in which five pairs of B. tabaci (F1) were released. The population buildup was recorded to categorize genotypes as resistant, moderately resistant, moderately susceptible or susceptible. The experiment was repeated with F2 and F3 generation whiteflies. Based on overall average score of three experiments, LD694 was rated as resistant; LK861, Supriya, RS2013, CNH911 and PA183 as moderately resistant; IS-376/4/1/20/72, NHH44, TxMaroon2-78, Bt 6304 and RS2098 as moderately susceptible; and F846 as susceptible. LD694 was found to be resistant in three consecutive generations of whitefly.