Abstract
In this research, the antibacterial activity and mechanism of daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens were evaluated. The minimum inhibitory concentration (MIC) of daphnetin on P. fluorescens and S. putrefaciens was 0.16 and 0.08 mg·mL−1, respectively. The growth curve test also showed that daphnetin had a good antibacterial effect. The results of intracellular component leakage and cell viability analysis illustrated that daphnetin destroyed the morphology of the cell membrane. According to scanning electron microscope and transmission electron microscope observations, the treated bacterial cells displayed obvious morphological and ultrastructural changes in the cell membrane of the two tested strains, whichconfirmed daphnetin’s damage to the integrity of the cell membrane. The findings indicated that daphnetin mainly exerted its antibacterial effect by destroying the membrane and suggested that it had good potential to be as a natural food preservative.
Highlights
Fish is a nutritious food source and is recommended because of its multiple nutritional values [1]
The bacterial suspensions of the test groups without visible bacteria growth were subcultured on nutrient agar to determine that the minimum concentration of daphnetin was defined as Minimum Bactericidal Concentration (MBC), which resulted in no colony growth at all [16]
Antibacterial Activity of Daphnetin. e antibacterial activity of daphnetin was evaluated by minimum inhibitory concentration (MIC) and MBC values
Summary
Received 7 October 2020; Revised 19 October 2020; Accepted 31 October 2020; Published 19 November 2020. The antibacterial activity and mechanism of daphnetin against Pseudomonas fluorescens and Shewanella putrefaciens were evaluated. E minimum inhibitory concentration (MIC) of daphnetin on P. fluorescens and S. putrefaciens was 0.16 and 0.08 mg·mL−1, respectively. E growth curve test showed that daphnetin had a good antibacterial effect. E results of intracellular component leakage and cell viability analysis illustrated that daphnetin destroyed the morphology of the cell membrane. According to scanning electron microscope and transmission electron microscope observations, the treated bacterial cells displayed obvious morphological and ultrastructural changes in the cell membrane of the two tested strains, whichconfirmed daphnetin’s damage to the integrity of the cell membrane. E findings indicated that daphnetin mainly exerted its antibacterial effect by destroying the membrane and suggested that it had good potential to be as a natural food preservative According to scanning electron microscope and transmission electron microscope observations, the treated bacterial cells displayed obvious morphological and ultrastructural changes in the cell membrane of the two tested strains, whichconfirmed daphnetin’s damage to the integrity of the cell membrane. e findings indicated that daphnetin mainly exerted its antibacterial effect by destroying the membrane and suggested that it had good potential to be as a natural food preservative
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